Technical Information

For several magnetic separation protocols, it is desirable to be able to track and quantify the magnetic particles.  For these applications, BioPAL has developed Trackable BioMagic™.  Trackable BioMagic is a standard iron oxide magnetic particle that is laced with europium.  As a result, researchers can track and quantify the fate of the magnetic particles under different experimental conditions either by time resolved fluorescent technology (TRF) or by neutron activation (NA).

Like TorrentDOTS™, the europium contained in the magnetic particle does not contain a TRF antenna.  As a result, researchers planning to use TRF readout systems will need to purchase BioPAL's releasing reagent.  This reagent is formulated to both (1) breakdown the iron oxide colloidal core and then (2) individually chelate an antenna onto the released europium atoms.  The degree of sensitivity and quantification achievable by TFR is dependent on many factors outside the control of BioPAL, such as the limitation of the customer's readout equipment and variation in sample preparation, i.e., sample size, density, viscosity, temperature, etc.  As a result, customers must evaluate these parameters.  Alternatively, BioPAl offers neutron activation analysis of samples-of-interest as an out-source service, i.e., customers send samples to BioPAl for analysis.  Neutron activation provides a sensitive and quantitative measurement that is matrix independent.  Trackable BioMagic has patent pending status.

The Products

1. Trackable BioMagic - Amino-Terminated:  1.5 µm amine-terminated, europium-laced iron oxide magnetic particles and are packaged in a 10 ml sealed bottle (50 mg/ml) suspended in distilled water/EDTA solution.
2. Trackable BioMagic - Immobilization Kit:  BioPAL has developed an immobilization kit for those researchers new to magnetic separation technology to customize the magnetic particles with their antibody.  The BioMagic immobilization kit consists of a suspension of 1.5 µm amine-terminated, europium-laced iron oxide magnetic particles, 25% solution of high purity glutaraldehyde, and instructions.
3. COMING SOON! Trackable BioMagic - Biotin:  1.5 µm biotin labeled, europium-laced iron oxide magnetic oxide particles and are packaged in a 5 ml sealed bottle (5 mg/ml) suspended in phosphate buffered saline (pH 7.5) containing 0.1% BSA with EDTA and sodium azide added.  One ml (5 mg) of BioMagic Biotin will bind >0.3 mg avidin.
4. COMING SOON! Trackable BioMagic - Streptavidin:  1.5 µm streptavidin labeled, europium-laced iron oxide magnetic particles and are packaged in a 5 ml or a 50 ml sealed bottle (5 mg/ml) suspended in phosphate buffered saline (pH 7.5) containing 0.1% BSA with EDTA and sodium azide added.  One ml (200 µl) of BioMagic Streptavidin will bind 2.0 µg biotin.
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   Download a PDF MSDS.

BioPAL offers a line of secondary antibody Trackable BioMagic europium-laced, iron oxide magnetic particles that are approximately 1.5 µm is size.  These products are ideally suited for use as a second antibody in radioimmunoassays, enzyme immunoassays and cell sorting that utilize an IgG primary monoclonal antibody.

1. COMING SOON! Trackable BioMagic - Goat anti-Mouse IgG:  1.5 µm Goat anti-Mouse IgG magnetic particles and are packaged in a 50 ml or a 500 ml sealed bottle (1 mg/ml) suspended in phosphate buffered saline (pH 7.5) with EDTA and sodium azide added.  One ml (1 µg) of BioMagic Goat anti-Mouse IgG will bind >0.2 mg of mouse IgG.
2. COMING SOON! Trackable BioMagic - Goat anti-Rabbit IgG:  1.5 µm Goat anti-Rabbit IgG magnetic particles and are packaged in a 50 ml or a 500 ml sealed bottle (1 mg/ml) suspended in phosphate buffered saline (pH 7.5) with EDTA and sodium azide added.  One ml (1 µg) of BioMagic Goat anti-Rabbit IgG will bind >0.1 mg of rabbit IgG.
3. COMING SOON! Trackable BioMagic - Goat anti-Rat IgG:  1.5 µm Goat anti-Rat IgG magnetic particles and are packaged in a 50 ml or a 500 ml sealed bottle (1 mg/ml) suspended in phosphate buffered saline (pH 7.5) with EDTA and sodium azide added.  One ml (1 µg) of BioMagic Goat anti-Rat IgG will bind >0.15 mg of rat IgG.
   Download a PDF price list.
   Download a PDF MSDS.

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