Technical Information

Time resolved fluorescent (TRF) technology is emerging as an important tool in drug discovery and diagnostics.  Based on our novel chelation and nanocolloid technology, BioPAL has developed a line of TRF reagents for research application.

TorrentDOTS™ are nanosized colloids consisting of a highly dense and stable lanthanide core and represents a major breakthrough in colloid technology.  The colloidal core of the particle is stabilized by a polymer.  The core is densely packed with many atoms of lanthanide label offering the highest potential sensitivity by TRF.  The polymer contains surface amine groups for covalent labeling of ligands and macromolecules.

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Unlike RadiantDOTS™, TorrentDOTS are not intrinsically fluorescent.  As a result, the final step of the assay procedure is the introduction of BioPAL's releasing reagent.  This reagent is formulated to both (1) breakdown the colloid core and then (2) activate the "torrent" of released lanthanide atoms.

The figure below is a calibration curve showing the degree of sensitivity achievable for TorrentDOTS and RadiantDOTS.  This work was preformed on a standard fluorescence spectrophotometer (340nm/615nm).  The blue squares plot the data for TorrentDOTS and the red circles plot the data for RadiantDOTS.  The figure demonstrates that RadiantDOTS can detect down to a few femto-mols, whereas TorrentDOTS can achieve enhanced sensitivity down to a few atto-mols.

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