Given the recent advance in biomedical research, a large number of lead drug candidates has been identified. In many cases, it is difficult to assay for the presence of the test reagent once it is introduced into an in vivo system. As a result, it has become increasingly important to develop methods to validate that the test reagent has reached its target. BioPAL's technology is ideally suited for this purpose.BioPAL can label the test reagent with a stable isotope. Moreover, BioPAL can label the reagent with more than one stable isotope to provide different labels for different time-points, thereby providing greater flexibility in the experimental design. The
investigator than uses the labeled reagent(s) in the in vivo system. At the close of the study, the researcher sends biopsies of the areas of interest and/or blood samples to BioPAL for quantitative isotopic analysis. Interested customers are encouraged to contact BioPAL's Technical Support Office with their questions (508-770-1190).
Alternatively, the researcher can co-inject the test reagent with an inert labeled compound. BioPAL has developed AccuJECT™ for this application. AccuJECT is a labeled nanocolloid which is directed to the Kupffer cells. Six different labels
are available. As a result, if AccuJECT enters the blood stream it will be quickly cleared via the liver. On the other hand, if AccuJECT is injected into tissue it will remain lodged at the injection site.
The applications for AccuJECT™ include the following:
- A Target Validation Marker:
For example, in cardiac research, this reagent can be co-injected along with a
test compound into live myocardial muscle. The presence of AccuJECT in tissue will ensure that the test compound was correctly administered and that the injection did not penetrate through the myocardium into the ventricle. In addition, other drug delivery systems, such as vectors and DNA vaccines, can be monitored.
- A Monitor for Tail Vein Injections:
Tail vein injections into small animals are not as facile as might appear. Before AccuJECT, injection errors were undetectable and unrecognized. As a result, potentially important drug candidates may have been prematurely dismissed due to a series of poor injections performed on just one day. The information provided by AccuJECT would establish a basis for eliminating improperly injected animals leaving a sample population free of false results due to misadministration.
- Training and Certification:
AccuJECT can be used as a teaching tool for technicians. The reagent can be used in a wide variety of protocols involving both intravenous (IV) and intraperitoneal (IP) injections. In addition, AccuJECT can be used to periodically certify trained technicians to ensure high scientific standards.
The Product
- AccuJECT
Six different stable-isotope labels are available. AccuJECT is a metal
colloid (300 nm) coated to direct the reagent to hepatocytes. This reagent
is ideally suited for research wherein catheter-directed drug delivery systems
are to be used.
Download a
PDF price list.
Download a PDF MSDS AccuJECT.
- Contaminant-Free, Sample Vials
BioPAL's polypropylene sample vials are cleaned to remove trace contamination and are calibrated for our activation and counting system. These vials must be used for the assay.
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- Saline-Free Buffer
BioPAL offers sansSaLine, a saline-free buffer, as a substitute for saline
or PBS in histochemical stains and for use to wash and clean samples in order to
minimize sodium background activity, thereby maximizing the sensitivity of the
measurement.
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a PDF price list.
Download a PDF MSDS sansSaLine.
- Assay Service
BioPAL charges a per-sample assay fee. The results of the assay are reported as the total disintegration per minute (dpm) measured in each sample for each corresponding
label.
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Download a PDF Assay Request Form.
Request for Additional Information and Pricing
