spacer
BioPAL, Inc.
   diamond HOME     diamond ABOUT US    diamond ADVANTAGES OF BIOPAL    diamond WHAT'S NEW    diamond CONTACT    

New Ways to Make Measurements in the Life Sciences.

spacer
  diamond photo
pixel



Molday ION
Molday IONTM  Family of Products

Technical Information

Molday ION™ is an iron oxide-based super paramagnetic (USPIO) contrast reagent having a colloidal size of 30 nm.  This reagent is classified as a darkening agent acting through the T2 relaxation process.  As an intravascular contrast agent, Molday ION has a blood half-life of several hours and can be used in a wide range of cardiovascular and vascular research.  In addition, we also offer Molday ION (-) that has a negative charge colloidal surface and is formulated for labeling cells using Poly-L-Lysine.

Molday ION C6Amine, as its name implies, is engineered to have terminal amino groups with different length spacers.  They are designed for cell labeling and to allow for efficient labeling with ligands, peptides, proteins, and nucleic acids (RNAi, DNA, etc.).  As a result, new biological properties can be conferred to the reagent, such as targeting to a specific receptor site.  These reagents are also classified as a darkening agent acting through the T2 relaxation process.

Molday ION Carboxyl Terminated nano-magnetite is specifically designed for conjugating your ligand, peptide, antibody, and protein using water soluble carbadiimide.  This T2 MRI contrast agent has a concentration of 2.5 mg Fe/ml, a size of ~30 nm, and a zeta potential of about -35 mV.  The nanoparticle is designed so that it is not bound to or internalized by cells allowing cell labeling exclusively from your conjugated ligand.  This product is well suited for cell targeting experiments!

Molday ION Rhodamine B is an iron oxide-based super paramagnetic contrast reagent having a colloidal size of 35 nm.  The colloidal particle is cross-linked and labeled with rhodamine B, which is a fluorescent dye.  As a result, this reagent can be visualized by both MRI and by fluorescence.  For MRI, this reagent is classified as a darkening agent acting through the T2 relaxation process.  For fluorescence, the rhodamine B label excitation wavelength is 555 nm and the emission is at 565-620 nm.  The reagent ideal for many cell labeling and tracking applications.  Download a PDF picture of labeled cells!

The Product Pricing

Molday ION, Molday ION Amine, Molday ION Carboxyl Terminated,  and Molday ION Rhodamine B, are both packaged in 2 ml sealed serum bottles.  Additional Molday ION products are listed on our price list.  NOT FOR HUMAN USE.
Download a PDF price list.
Download a PDF MSDS.
 

 

Applications and Functionalities

Catalog No.

Name

Application

Functionality

CL-30Q02-2

Molday ION

Blood pool agent

Hydroxyl

CL-30Q02-6

Molday ION (-)

Cell labeling with PLL

Hydroxyl

CL-50Q02-6A

Molday ION C6Amine

1.  Cell labeling
2.  Conjugation

Amino

CL-30Q02-6C

Molday ION Carboxyl

1.  Cell labeling with PLL
2.  Conjugation

Carboxyl

CL-50Q02-6A-50

Molday ION Rhodamine B

Cell labeling

Rhodamine B

Physical properties

Catalog No.

Name

~Size
nm

~Zeta potential
mV

CL-30Q02-2

Molday ION

30

-4.8

CL-30Q02-6

Molday ION (-)

30

-15

CL-50Q02-6A

Molday ION C6Amine

35

48

CL-30Q02-6C

Molday ION Carboxyl

35

-38

CL-50Q02-6A-50

Molday ION Rhodamine B

35

31

Volume, Concentration and Price

Catalog No.

Name

Volume
ml

Concentration
mg Fe/ml

CL-30Q02-2

Molday ION

2

10

CL-30Q02-6

Molday ION (-)

2

10

CL-50Q02-6A

Molday ION C6Amine

2

5

CL-30Q02-6C

Molday ION Carboxyl

2

2.5

CL-50Q02-6A-50

Molday ION Rhodamine B

2

2

 

Helpful Download Files

Application Notes:
Receptor mediated endocytosis: Conjugation of spermidine to CL-30Q02-6C, a carboxyl containing USPIO
Download this PDF Application.

Cell labeling and tracking by MRI is a growing area of interest.  BioPAL provides a general method to label and fix cells using Molday ION product line.
Download a PDF method to label cell.
Download a PDF method to fix cell.

BioPAL provides a method to label poly-l-lysine on to Molday ION (-) for cell labeling.
Download a PDF method for poly-lysine.
Download a PDF Cell labeling method and long-term stability.

BioPAL provides a method to stain cells with Prussian Blue to visualize the presence of iron.
Download a PDF method for Prussian Blue Staining.

 

References

Download a PDF of a recent publication - 1
Download a PDF of a recent publication - 2

1.  J Clin Invest 2008 118(5):1965-1973.
2.  Clin Exp Metastasis 2009 26:403-414.
3. Neuroimage 2009 Jul 17 [Epub ahead of print]

 

Request for Additional Information and Pricing