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New Ways to Make Measurements in the Life Sciences. |
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Technical Information FIT Molecular Imaging and Tracking Technology (FIT-MITT) provides researchers with a universal method to image by MRI and quantify by immunoassay compounds-of-interest in in vivo systems. By sequential sampling, the kinetic clearance and/or uptake of the compound can be determined. FIT-MITT is a two component process. First, using BioPAL's labeling kit or labeling service, the researcher covalently labels the compound-of-interest with either Gd-DTPA or Gd-DOTA tags. Second, using BioPAL's FIT-MITT kit, the tag on the compound-of-interest is measured in collected samples, such as blood or urine, using immunoassay technology, or using BioPAL's antibody reagent, the tag on the compound-of-interest is evaluated using standard immuno-histochemisty techniques. Examples of compounds that can be labeled and tracked by FIT-MITT include, but are not limited to: peptides, proteins, antibodies, liposomes, nucleic acids (DNA, RNA, RNAi or siRNA probes) or new drug entities. Applications include drug discovery, drug delivery, molecular biology, molecular imaging, in vivo and in vitro high throughput screening This universal tagging technology offers many advantages (1) the FIT-MITT is a rapid and low cost method to evaluate and screen new compounds, (2) immunoassay is a widely used and available technology, (3) the test is highly sensitive, (4) the chelate tags (Gd-DTPA or Gd-DOTA) are stable in vivo, (5) two different labels are available and can be quantified in the same sample, (6) both Gd-DTPA and Gd-DOTA have an exemplary safety profile and are clinically used as MRI contrast reagents, (7) this approach allows researchers the ability to image labeled compounds using MRI or (8) using immuno-histochemisty techniques. The sensitivity of each kit to the tag is, as follows: Gd-DTPA 0.5 picograms/ml; Gd-DOTA 5 femtograms/ml.
Above is a plot of the ELISA data using gadolinium-labeled albumin. The solid circles are Gd-DTPA labeled albumin and the hollow squares are Gd-DOTA labeled albumin. In general, the Gd-DOTA FIT-MITT kit is 100x more sensitivity than the Gd-DTPA FIT-MITT kit. However, the sensitivity of either test is greatly dependent of the number of Gd-units that can be covalently attached to the compound-of-interest. FOR RESEARCH USE ONLY.
The Product Gadolinium Labeling Kits FIT-MITT detection Kits (Patents Pending) The Laboratory Service BioPAL offers custom protein labeling services to assist researchers that wish to out-source the labeling procedure. | |||
